Lumbar puncture in adult:
  • Patient is lying on side with maximally flexed spine. A line is taken between highest points of iliac spine at L4 level. Skin locally anaesthetized, and lumbar puncture needle with trocar inserted carefully between L3 and L4 spines. 
  • Needle courses through skin fat, supraspinous and interspinous ligaments, ligamentum flava, epidural space, dura, arachnoid, subarachnoid space to release CSF


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